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Microglia cell count. Eight evenly spaced frozen sections that encompassed the entire SN were stained with anti-CD68, a marker for activated microglia. Total count of CD68-positive cells was counted unilaterally in the region of SN of wt (n = 11), casp1−/− (n = 6), and IL-1ra−/− (n = 6) mice and analyzed by one-way ANOVA followed by Newman-Keuls post hoc test. Error bars represent standard errors. **P < 0.01 vs. wt; ***P < 0.001 vs. wt; +++ P < 0.0001 vs. casp1−/−
