Figure 8. Wortmannin fails to inhibit OT neurons in aCSF containing myo‐inositol.

Slices were preincubated in aCSF containing 1 mm myo‐inositol, an important precursor molecule for PIP₂ synthesis. When the slice was transported to the stage for patch clamp, the aCSF perfused onto the slice contained 300 μm myo‐inositol during recording. A, voltage clamp trace of an OT neuron before and after application of wortmannin. Wortmannin had no effect on the OT I _AHP when the cells were bathed in 300 μm myo‐inositol. B, summary data for OT neurons. There were no significant differences in amplitude of current at 100 ms and 1000 ms after the pulse (n = 7, P > 0.05). [Color figure can be viewed at wileyonlinelibrary.com]