Figure 2. Time course responses of p38 MAPK phosphorylation.

A, phosphorylation of p38 MAPK in P‐UAECs upon treatment with E₂β, 2‐OHE₂, 4‐OHE₂, noradrenaline (NA) and adrenaline (ADR) (all 0.1 nm) over the course of 24 h. Phosphorylation of p38 increased gradually over time with 2‐OHE₂, 4‐OHE₂, E₂β, NA and ADR. Graphs represent the means ± SEM (Student‐Newman‐Keuls test, P < 0.05 was considered statistically significant). B, representative blots of phosphorylated (Phospho), total ERK1/2 MAPK and β‐actin (n = 3).