Figure 1. The effects of cytosolic ATP and luminal Ca²⁺ on RyR1 channels from Tric‐a KO mice with K⁺ as the permeant ion.

Representative single‐channel recordings of RyR1 from WT (A) and Tric‐a KO (B) mice under control conditions (10 μm cytosolic and luminal Ca²⁺), after subsequent addition of 1 mm cytosolic ATP (second traces) and subsequent increasing concentrations of luminal Ca²⁺ (100 μm and 1 mm as indicated). The bar charts below illustrate the mean data for RyR1 channels from WT (white) (C) and Tric‐a KO (pink) (D) skeletal muscle under control conditions, in the presence of ATP and increasing concentrations of luminal Ca²⁺. Values are the mean ± SEM (n = 6–10; ^* P < 0.05, ^** P < 0.01). The holding potential was −30 mV. O and C indicate the open and closed channel levels, respectively.