Figure EV2. LC3 and ATG16L1 reside at ER‐PM contact sites under starvation conditions.

• A, B
  Confocal microscope images and 3D reconstructions of HeLa cells co‐transfected with GFP‐E‐Syt2 and RFP‐Sec61β and immunostained for LC3 or ATG16L1 and DAPI. Arrowheads denote LC3 or ATG16L1 puncta near the E‐Syt2‐positive niche of the ER. Scale bars, 5 and 2.5 μm (magnified areas).
• C
  Quantification of LC3 and ATG16L1 co‐distribution with E‐Syt2 at basal plan of the cell n = 80 cells. Mean ± s.e.m. shown.
• D
  Immunogold electron micrographs of starved HeLa cells, showing co‐distribution of the myc‐E‐Syt2 and anti‐LC3 antibody at ER (empty arrowheads) and PM (black arrowheads) juxtaposition sites. Scale bar, 250 nm.