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. 2017 May 26;36(14):2018–2033. doi: 10.15252/embj.201797006

Figure EV4. Overexpression of E‐Syt2 and E‐Syt3 enhances autophagy.

Figure EV4

  1. An electron micrograph of GFP‐E‐Syt2‐expressing HeLa cells starved for 1 h, showing an increased number of autophagic structures (arrowheads) compared to starved control cells. Scale bar, 1 μm. Wortmannin (wort, 100 nM) was used as a negative control, and autophagic structures were counted in 15 μm2 areas (n = 10 cells).
  2. Proteolysis analysis showing an increased protein degradation rate in GFP‐E‐Syt2‐ and GFP‐E‐Syt3‐expressing cells (n = 3). 3‐methyladenine (3‐MA) was used at 10 mM.
Data information: Means ± s.e.m. are plotted. *P < 0.05, **P < 0.01, ***P < 0.001, unpaired two‐tailed t‐test.