Figure EV5. Autophagosome maturation is not affected in E‐Syt‐deficient cells.

• A, B
  HeLa cells stably transfected with mRFP‐GFP‐LC3 and treated with siE‐Syts have fewer autophagosomes, but autophagosomes have normal functionality, as evidenced by (A) microscopy and (B) by RFP⁺/GFP⁺ and RFP⁺/GFP⁻ LC3‐puncta counting. Cells treated with Bafilomycin A1 (+BAF. A1) and siCTRL were used as a positive functionality control and showed decreased autophagosome maturation (i.e. reduced RFP⁺/GFP⁻ LC3‐puncta compared to siCTRL‐treated cells) as expected (n = 3). ***P < 0.001, unpaired two‐tailed t‐test. Scale bar, 10 μm. Plotted are mean ± s.e.m.