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A, B
HeLa cells were treated with control siRNA (siCTRL) or with siRNAs targeting mRNAs encoding each of the E‐Syts (siE‐Syts). (A) Western blots of cell lysates. Actin is used as a loading control. (B) E‐Syts mRNAs were quantified. Means ± s.d. are plotted (n = 3).
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C
HeLa cells stably transfected with GFP‐LC3 and treated with siE‐Syts or siCTRL were not treated (−BAF. A1) or were treated with Bafilomycin A1 (+BAF. A1). Representative images are shown. Empty arrowheads indicate peripheral LC3 puncta. Scale bars, 10 μm.
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D
Quantification of experiments shown in panel (C) (n = 3; 20 cells per condition). Means ± s.e.m. are plotted.
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E
HeLa cells treated with control siCTRL or with siE‐Syts were grown in complete medium or were starved for 1 or 4 h, and cells lysates were subjected to Western blot for indicated proteins.
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F
Quantification of Western blot shown in panel (E), with 20 cells analysed per condition (n = 5).
Data information: NS, non‐significant, *
P < 0.05, **
P < 0.01, ***
P < 0.001, unpaired two‐tailed
t‐test.
