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. 2017 May 3;36(14):2061–2072. doi: 10.15252/embj.201696189

Figure EV1. Isolation of Bacillus subtilis 100S and sequence alignments of BsHPF with EcHPF‐NTD and CaCTD.

Figure EV1

  1. Sucrose density gradient profile of B. subtilis extract from late log phase cells, with 30S, 50S, 70S, 100S, and polysome peaks indicated.
  2. Negative stain electron microscopy images of purified Bs100S from (A), with selected 70S dimers circled in yellow.
  3. PROMALS3D (Pei et al, 2008) sequence alignment of BsHPF‐NTD with Escherichia coli HPF (PDB 4V8H)(Polikanov et al, 2012) that was used to generate the homology model for BsHPF‐NTD.
  4. PROMALS3D (Pei et al, 2008) sequence alignment of BsHPF‐CTD with Clostridium acetobutylicum HPF‐CTD (CaCTD; PDB ID 3KA5) that was used to generate the homology model for BsHPF‐CTD.
Data information: In (C) and (D), fully conserved residues are indicated with “9” and are bold in the Consensus_aa, whereas similar residues are indicated with a “+”. Consensus_ss indicates β‐sheet (e) and helical (h) regions.