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. 2017 Jun 12;36(14):2047–2060. doi: 10.15252/embj.201796664

Figure 2. Inhibition of XE activity by a model nascent leading strand is overcome by the addition of RPA .

Figure 2

  1. Nuclease activity of XE on a “simple fork” with increasing concentration of RPA or SSB (Escherichia coli).
  2. Quantification of intact substrate and incision products as a percentage of initial substrate as in (A). Error bars represent SEM, n = 3.
  3. A schematic representation of 40 nM XE activity on a “simple fork” in the absence (left) or presence (right) of 80 nM RPA.
  4. Nuclease activity of XE on fork substrates with increasing length of a model nascent leading strand (simple fork; −9; −3; −2; −1; 0 nt from the fork junction) in the presence or absence of 80 nM RPA.
  5. A schematic representation of XE activity in the presence and absence of RPA as in (D).