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. 2017 Jun 12;36(14):2126–2145. doi: 10.15252/embj.201696179

Figure EV4. Assessment of overall mitochondrial function and muscle health in mOPA1/FGF21 DKO mice.

Figure EV4

  1. Representative immunoblots and densitometric quantification of OPA1 normalized to GAPDH in gastrocnemius muscle from 12‐week‐old WT and mOPA1/FGF21 KO mice (n = 3).
  2. BN‐PAGE in gastrocnemius muscle of 12‐week‐old mice (n = 4).
  3. mtDNA copy number in gastrocnemius muscle of 12‐week‐old mice (n = 3–4).
  4. Maximally stimulated succinate‐supported mitochondrial respirations in soleus from 20‐week‐old mice (n = 4–6).
  5. Succinate‐supported ATP synthesis rates in soleus from 20‐week‐old mice (n = 4–5).
  6. Maximally stimulated succinate‐supported mitochondrial respirations in soleus from 40‐week‐old mice (n = 5).
  7. Measurements of grip strength in 40‐week‐old mice (n = 3).
  8. Body weight and body composition in 20‐week‐old WT and DKO mice (n = 5–9).
  9. Cross sections of gastrocnemius muscle from 20‐week‐old mice stained with wheat‐germ agglutinin (WGA). Scale bar = 50 μm.
  10. Measurements of muscle fiber diameter in gastrocnemius muscle from 20‐week‐old mice (n = 3).

tPData information: Data are expressed as means ± SEM. Significant differences were determined by Student's t‐test, using a significance level of P < 0.05. (*) Significantly different vs. WT mice. Source data are available online for this figure.