Fig. 3. (a) Two desthiobiotin-labelled DNA strands conjugated to FAM or DABCYL were mixed with SA or BSA in different orders or at different temperatures; fluorescence decreases were then measured. (b) Left panel: data from different mixing orders. Right panel: data from different temperatures; SA was used as the target except in 5 where BSA was used as a negative control. The experimental conditions were the same as those for Fig. 2 except for the mixing order and temperature. Error bars (standard deviation, SD) are based on three replicates of each experiment.
