Fig. 4. Verification of the target-induced equilibrium shift, determined by a fluorescence decrease. Fluorescence values were normalized to the “no protein” experiment. AD-1 and LD-1: 200 nM; BD-1: 1.6 μM; proteins: 400 nM. The experimental procedures were the same as those for Fig. 2. Error bars (standard deviation, SD) are based on three replicates of each experiment.
