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. Author manuscript; available in PMC: 2018 Jul 1.
Published in final edited form as: Exp Hematol. 2017 Apr 24;51:25–35.e6. doi: 10.1016/j.exphem.2017.04.006

Figure 4. CFU output of hemogenic endothelial clones.

Figure 4

A) % of individual colonies from OP9/ or AA-EC/ HE co-cultures capable of re-plating into semi-solid media supplemented with hematopoiesis promoting cytokines. Bars represent the average from five independent experiments, three of them performed in parallel. A total of 86 and 59 colonies were plated respectively from OP9/ and AA-EC/ HE co-cultures. Error bars indicate standard deviation. B–F) Colonies with replating-ability were further analyzed for their CFU type composition. B) Total absolute number of CFUs generated by colonies collected from OP9 or AA-EC HE co-cultures. C) Total absolute number of CFU-G/M/GM (granulocyte and monocyte colonies) generated by colonies collected from OP9/ or AA-EC/ HE co-cultures. D) Total absolute number of CFU-Es (erythroid) generated by colonies collected from OP9/ or AA-EC/ HE co-cultures. E) Total absolute number of CFU-GEMMs (mixed colonies of granulocytes, erythrocytes, macrophages, and megakaryocytes) generated by colonies collected from OP9/ or AA-EC/ HE co-cultures. For B–E, each circle represents the output of an independent colony collected from OP9 (white circles) and AA-EC (black circles) co-cultures. F) % of primary colonies that generated >seven CFU-GEMMs, 500 or 1000 CFU-G/M/GMs, and more than 500 or 1000 CFU-Es. *, P < 0.1; **, P < 0.05; ***, P< 0.001; n.s.: not statistically significant.