Table 3.

Summary of the results of the behavioral outcomes used for phenotype analysis in PS19 mice

Behavior	Test	Dependent variable	Genotype	Treatment	Mean ± SEM	p value	Interpretation
Locomotion	AC 1	Ambulatory distance (cm)	PS19 nTg	Vehicle	83.64 ± 5.01	—	PS19 Tg mice displaya hyperactive phenotypeevidenced by increasedlocomotor activity
				ISRIB	97.50 ± 6.89	ns
			PS19 Tg	Vehicle	82.65 ± 6.73	ns
				ISRIB	105.48 ± 5.87	ns
	AC 2	Ambulatorydistance (cm)	PS19 nTg	Vehicle	79.88 ± 4.43	—
				ISRIB	78.79 ± 5.60	ns
			PS19 Tg	Vehicle	98.85 ± 7.06	ns
				ISRIB	103.98 ± 5.26	p < 0.05
	AC 3	Ambulatorydistance (cm)	PS19 nTg	Vehicle	75.09 ± 5.16	—
				ISRIB	70.62 ± 4.98	ns
			PS19 Tg	Vehicle	90.88 ± 8.72	ns
				ISRIB	95.82 ± 6.62	p < 0.05
	AC 4	Ambulatorydistance (cm)	PS19 nTg	Vehicle	79.78 ± 5.28	—
				ISRIB	78.59 ± 5.85	ns
			PS19 Tg	Vehicle	86.06 ± 10.40	ns
				ISRIB	92.07 ± 6.42	ns
Anxiety-likebehavior	EPM	Open arm duration (%)	PS19 nTg	Vehicle	10.67 ± 1.55	—	PS19 Tg miceexhibit reduced anxiety-like behavior
				ISRIB	14.14 ± 2.23	ns
			PS19 Tg	Vehicle	24.99 ± 3.53	p < 0.001
				ISRIB	22.80 ± 2.91	p < 0.01
Spatial acquisition learning	MWM D1	Escape latency (s)	PS19 nTg	Vehicle	52.26 ± 3.86	—	PS19 Tg mice displayimpairments in spatiallearning with modestrestoration by ISRIB
				ISRIB	60.11 ± 3.52	ns
			PS19 Tg	Vehicle	83.22 ± 1.94	p < 0.0001
				ISRIB	74.89 ± 6.55	p < 0.01
	MWM D2		PS19 nTg	Vehicle	30.15 ± 3.90	—
				ISRIB	33.01 ± 3.89	ns
			PS19 Tg	Vehicle	58.06 ± 6.04	p < 0.0001
				ISRIB	55.05 ± 6.61	p < 0.01
	MWM D3		PS19 nTg	Vehicle	19.38 ± 2.50	—
				ISRIB	24.03 ± 3.61	ns
			PS19 Tg	Vehicle	43.58 ± 6.60	p < 0.0001
				ISRIB	37.15 ± 4.50	ns
	MWM D4		PS19 nTg	Vehicle	18.32 ± 3.21	—
				ISRIB	22.36 ± 4.50	ns
			PS19 Tg	Vehicle	43.08 ± 7.72	p < 0.001
				ISRIB	28.38 ± 6.16	ns
	MWM D5		PS19 nTg	Vehicle	12.56 ± 1.32	—
				ISRIB	16.87 ± 3.59	ns
			PS19 Tg	Vehicle	41.98 ± 6.54	p < 0.0001
				ISRIB	26.54 ± 4.04	ns
Spatial memory recall	Prove test 24 h	Quadrantduration (%)	PS19 nTg	Vehicle	−24.45 ± 2.71	p < 0.0001	PS19 Tg mice display impaired spatial memory recall
				ISRIB	−21.54 ± 3.55	p < 0.0001
			PS19 Tg	Vehicle	−6.43 ± 6.89	ns
				ISRIB	−4.26 ± 5.28	ns
	Prove test 72 h		PS19 nTg	Vehicle	−20.84 ± 3.07	p < 0.0001
				ISRIB	−15.33 ± 4.20	p < 0.01
			PS19 Tg	Vehicle	2.57 ± 6.76	ns
				ISRIB	−2.76 ± 5.70	ns
Fear learningand memory	PA, trainingvs testing	Latency to cross (s)	PS19 nTg	Vehicle	117 ± 22	p < 0.0001	PS19 Tg mice do not exhibit deficits in fear-based learning and memory
				ISRIB	137 ± 24	p < 0.0001
			PS19 Tg	Vehicle	155 ± 28	p < 0.0001
				ISRIB	100 ± 22	p < 0.001

Comparisons made to nTg vehicle control. AC, activity chamber; D1-5, day 1-5.