Figure 3.

(A, B) PLA–glass device with sealed fluidic channels, suitable for microscopic inspection (see the Supporting Information, Figure S3, for the dimensions). The glass slide is incorporated into a single 3D-printed PLA part. The glass is inserted during the print by pausing the print. (C) Transparent PLA was used, which gives semitransparency through a limited thickness. The thickness of the top PLA layer through which the blue solution is being visualized is 0.8 mm, which is clearly too thick for a clear image of the microchannel (1 mm wide and high). (D) The blue solution in the same printed channel can be clearly imaged under the microscope when viewed through the glass bottom. There appears to be a bit of leakage along the edges of the PLA channel where it contacts the glass slide.