Fig. 3.

Cys31 is required for activation of LCAT by compound A. Conditioned media from cells transfected with WT-LCAT (Glukhova et al., 2015) and C31G and C184G mutants were incubated with the indicated concentrations of compound A (x-axis) and then tested for LCAT activity with MUP as the substrate. Results are normalized to untreated activity levels for each LCAT variant. Results represent means ± S.D. of at least triplicate measurements. ***P < 0.005 compared with WT-LCAT treated under the same conditions.