Skip to main content
. 2017 Jul 12;8:16037. doi: 10.1038/ncomms16037

Figure 1. Lymphoproliferative autoimmunity in immune cell-specific Cic null mice.

Figure 1

(a,b) Spleen weights (a) and the numbers of total splenocytes, B220+ B, CD3+ T, CD4+ T, CD8+ T, macrophages (CD11b+CD11c−F4/80+) and dendritic cells (CD11b+CD11c+MHCII+) (b) in 10-week-old Cicf/f (WT) and Cicf/fVav1-Cre (cKO) mice. (c) FACS analysis showing the proportion of CD44loCD62Lhi naïve T cells and CD44hiCD62Llo effector/memory T cells in spleen of Cicf/f and Cicf/fVav1-Cre mice. All data are representative of two independent experiments. Data are presented as box-and-whisker plots. n=4–5 per each genotype. (d) Serum levels of total IgG, IgG1, IgG2a and IgM in 12-week-old Cicf/f and Cicf/fVav1-Cre mice were measured by ELISA. (e) Images of dissected spleens (SPL) and draining lymph nodes (LN) from Cicf/f and Cicf/fVav1-Cre mice at the age of 12 months. The graph shows average weights of spleens from the Cicf/f and Cicf/fVav1-Cre mice. M, male; F, female. (f) Serum levels of anti-dsDNA antibody in the 12-month-old Cicf/f and Cicf/fVav1-Cre mice were measured by ELISA. The graphs in a,b,d–f show data as mean±s.e.m. and each dot in graphs represents an individual mouse. *P<0.05, **P<0.01 and ***P<0.001 (two-tailed two-sample unequal variance Student t-test). (g) Immune cell infiltration in liver and lung from the 12-month-old Cicf/fVav1-Cre mice. Tissue sections were stained with haematoxylin and eosin (H&E). Arrows indicate immune cell infiltrates. (h) H&E-stained kidney sections showing glomerulonephritis in the 12-month-old Cicf/fVav1-Cre mice. (i) Immunostaining for IgG deposition in kidney glomeruli of the 12-month-old Cicf/fVav1-Cre mice. Representative images from two independent experiments are shown. (g–i) Scale bars, 100 μm.