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Primer sequences and conditions for RT-PCR.
| Target genes | Primer sequence (5ʹ-3ʹ) | Annealing Tm (°C) | PCR cycles |
|---|---|---|---|
| GAPDH | F: CACTCACGGCAAATTCAACGGCA | 60 | 30 |
| R: GACTCCACGACATACTCAGCAC | |||
| iNOS | F: CCCTTCCGAAGTTTCTGGCAGCAG | 60 | 27 |
| R: GGCTGTCAGAGCCTCGTGGCTTTGG | |||
| COX-2 | F: CACTACATCCTGACCCACTT | 55 | 30 |
| R: ATGCTCCTGCTTGAGTATGT | |||
| TNF-α | F: TGCCTATGTCTCAGCCTCTTC | 55 | 30 |
| R: GAGGCCATTTGGGAACTTCT |
