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. Author manuscript; available in PMC: 2017 Jul 14.
Published in final edited form as: Breast Cancer Res Treat. 2016 May 13;157(2):253–265. doi: 10.1007/s10549-016-3829-5

Figure 4. MCF-7 Y537S cells show the lowest anti-proliferative response to Tam through increased interaction between IGF1R/ER.

Figure 4

a, b, and c, Immunoblot analysis to detect expression of IGF1Rβ, pIRS-1, IRS-1 and ER in input extracts; GAPDH was used as a loading control (a). Coimmunoprecipitation assays of IGF1R/ER interactions (b and c). Duolink staining (red) demonstrated binding between IGF1R and ER in MCF-7(d) and ZR-75B cells (e). Images are representative of three different experiments.