Production of cAMP in response to activation of the human FSH receptor in stably transfected HEK-293 cells was measured across the indicated concentrations of follitropin delta (open circles, blue), follitropin alfa (open squares, red) or no recombinant FSH (open triangles, black) with increasing luminescence (RLU) proportional to increasing cAMP present. Each concentration of rFSH proteins was tested in quadruplicate, with the mean and s.d. shown.