Skip to main content
. Author manuscript; available in PMC: 2017 Jul 14.
Published in final edited form as: J Dermatol Sci. 2016 Dec 12;85(3):197–207. doi: 10.1016/j.jdermsci.2016.12.010

Fig. 5.

Fig. 5

A mixture of pathogenic and non-pathogenic anti-desmoglein 1 (Dsg1) IgG monoclonal antibodies (mAbs) induced Dsg1 clustering which enhanced loss of cell adhesion in primary keratinocytes. A: Immunofluorescence imaging of primary keratinocytes. Keratinocytes were incubated in 0.5 mM calcium-containing medium for 24 h to induce Dsg1 expression and then treated anti-Dsg1 IgG mAbs for 24 h. Keratinocytes treated with the mixture of PF1-8-15 IgG and PF1-2-6 IgG (PF1-8-15 IgG + PF1-2-6 IgG) showed Dsg1 clustering. Bar = 50 μm. B: Dissociation assay corresponding to the immunofluorescence imaging series (n = 6 per group). PF1-8-15 IgG + PF1-2-6 IgG showed higher dissociation index than the mixture of PF1-8-15 IgG and PF1-2-6 scFv (PF1-8-15 IgG + PF1-2-6 scFv) or PF1-8-15 IgG alone. Photos show the fragments condition of each well. Data are mean SEM. *p < 0.05.