Table 2.

Western Blot Analysis. Protein samples concentration were adjusted with buffer (80%:20%, Laemmle buffer 5X: b-mercaptoethanol) to 3µg/mL. Antibodies were diluted with Tween OBB 0.1%, probed with goat anti-mouse, goat anti-rabbit (IR-Dye 670 or 800cw labeled secondary antisera, 1:15,000 dilution) in 0.1% Tween OBB for 1 h at room temperature. Washes were repeated three times in 1X PBS-T (0.1% Tween 20). Membranes images were obteined using LiCor Odyssey scanner. (NC= Negative control , PC = Positive control, Experimental NBQ-48 and ABQ-48 compounds.

Antibody	NC	PC	NBQ-48	ABQ-48
Caspase 3 (1:1000)	-	+	+	+
Caspase 8 (0.3ugm/ml)	-	+	-	-
AIF (1:1000)	-	+	-	+