Figure 4.

Inhibition of MGMT activity by BGS, DABGS, and IOPH-pBGS. Suppression of MGMT activity in SF767 cells treated with BG, BGS or DABGS at 10 and 20 μM Cells were harvested 2 h and 24 h after inhibitor addition. MGMT activity was determined from protein extractions by quantitating transfer of radioactivity from O6-[3H]methylguanine to MGMT. Data represent the results of a single preparation of IOPH-pBGS and are representative of results obtained from independent preparation of IOPH-pBGS.