Figure 5.

Assessment of fibrosis, PSC activation, and apoptosis in the pancreas of high-fat-fed Stk25 transgenic and wild-type mice. (A) Representative images stained with Picrosirius Red and counterstained with Fast Green. Quantification of Picrosirius Red staining. (B) Representative images stained with antibodies for α-SMA; nuclei stained with DAPI (blue). White arrows indicate α-SMA-positive cells within the islet. Quantification of α-SMA staining. (C) Representative images stained with TUNEL. Quantification of TUNEL-positive staining. Scale bars, 25 µm. Data are mean ± s.e.m. from 5–8 mice/genotype. *P < 0.05; **P ≤ 0.01. HFD, high-fat diet; TG, transgenic; WT, wild-type.