Fig. 1.

Gating strategy for defining IFN-γ and IL-4 producing CD4⁺ and CD8⁺ T cells. Left panel represents scatter profile of PBMCs gated on lymphocytes with forward scatter (FSC) on x-axis and side scatter (SSC) on y-axis. Lymphocytes stained with fluorescently conjugated anti-CD3 PECy5 and CD8 ECD were gated on CD3⁺ T cells as shown in a histogram (middle panel). The CD3⁺ T cells were further gated on CD8⁺ T cell subset and CD8^- population was taken as CD4⁺ (right panel). Dot plots show CD4⁺ IFN-γ⁺ and CD8⁺ IFN-γ⁺ population (top right panel) and CD4⁺ IL-4⁺ subset (bottom right panel). The CD8 T cell subset producing IL-4 was negligible