Fig. 2.

HPV antigen-specific CD4 and CD8 T cell immune responses in wart patients. PBMCs obtained from the patient were stimulated with recombinant HPV-6 or HPV-11 antigen for 24 h in presence of Brefeldin A to block secretion of cytokines. Intracellular cytokine staining was performed to determine IFN-γ⁺ and IL-4⁺ producing CD4 and CD8 T cell subsets. Unstimulated PBMCs served as a negative control. a Representative dot plots showing IFN-γ producing CD4 and CD8 T cell population under conditions as indicated. b Representative dot plots showing CD4⁺ IL-4⁺ T cell subset under the indicated conditions