Table 3.

FACS analysis of cell surface binding of Grem2 WT and mutants

	Concentration (nM)	MFIa
Figure 5A
Control cells	–	4.8
Grem2 WT	50	29.2
	100	43.3
	200	74.0
	400	111.0
Figure 5B
Control cells	–	4.8
Grem WT	50	29.2
K111A/K112A/K151A/K153A	50	20.2
K147A/K148A/K151A/K153A	50	13.2
Figure 5C
Control cells	–	3.3
Grem2 WT	50	5.9
K111A/K112A/K151A/K153A	50	3.8
K147A/K148A/K151A/K153A	50	3.7
Figure 5D
Control cells	–	2.2
Grem2 WT	50	4.2
K111A/K112A/K151A/K153A	50	3.8
K147A/K148A/K151A/K153A	50	3.6

Abbreviations: MFI, mean fluorescence intensity.

^aMFI was determined using a 488 nM laser.