FIG. 4.

(A) Flow cytometry histograms showing the proportions of AT-MSCs and BM-MSCs (upper and lower panels, respectively) that are positive for staining with the antibodies CD146-AF647, NG2-APC, and αSMA (with secondary AF405 conjugated). Isotype controls are shown by the gray peak and unstained curves are omitted for simplicity. (B) Results of qPCR analysis of CD146, NG2, and PDGFRβ in AT-MSCs and BM-MSCs at passages 2 (P2) and 8 (P8). All results are shown as mean ± SEM; n = 3 animals. *P < 0.05.