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. 2017 Jul 1;26(13):973–985. doi: 10.1089/scd.2016.0331

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© María Ciria et al. 2017; Published by Mary Ann Liebert, Inc.

This article is available under the Creative Commons License CC-BY-NC (http://creativecommons.org/licenses/by-nc/4.0). This license permits non-commercial use, distribution and reproduction in any medium, provided the original work is properly cited. Permission only needs to be obtained for commercial use and can be done via RightsLink.

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FIG. 1. — HIF-1α overexpression in MSCs. (A) pWPI GFP and HIF-GFP lentiviral vectors. (B) Human dental pulp MSC lentiviral labeling with pWIPI-GFP (MSCs) and pWIPI-HIF-GFP (HIF-MSCs) detected by flow cytometric analysis of GFP. (C) HIF-1α gene expression levels detected by RT-qPCR in HIF-MSCs (gray) relative to MSCs (black) and expressed as mean ± SD of fold change. (D) Levels of HIF-1α protein as detected by western blotting with an anti-HIF-1α antibody and quantification by densitometry of exposed films in HIF-MSCs (gray) relative to MSCs (black); α-tubulin was used as a protein loading control. (E) Confocal microscopy (representative images) of MSCs and HIF-MSCs showing HIF-1α nuclear localization. Scale bar: 30 μm in upper and lower left panels and 5 μm in the remaining panels. Data represent mean ± SD of three independent experiments. *P < 0.05, ***P < 0.001. GFP, green fluorescent protein; HIF1α, hypoxia-inducible factor 1 alpha; MSCs, mesenchymal stem cells; RT-qPCR, real time quantitative PCR; SD, standard deviation.