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. 2017 Jul 1;26(13):973–985. doi: 10.1089/scd.2016.0331

FIG. 2.

FIG. 2.

HIF-1 enhances Notch signaling in MSCs. (A) RT-qPCR analysis of Notch downstream genes Hes1, Hey1, and Hey2 in MSCs (black bars) and HIF-MSCs (gray bars) cultured with (+) or without (−) recombinant Jagged1 (Jag) and RO4929097 (gamma-secretase inhibitor) as negative control. Data represent mean ± SEM of three independent experiments. (B) Representative western blots and quantification of Notch ligands Jagged1, Jagged2, and Delta-like family (Dll1, Dll3, and Dll4) in MSCs and HIF-MSCs. Expression levels of Notch ligands were quantified by densitometry of exposed films (black bars for MSCs and gray bars for HIF-MSCs). Data represent mean ± SEM of three independent experiments. (C) RT-qPCR analysis of basal expression of principal downstream genes of Notch pathway signaling (Hes1, Hey1, and Hey2) in confluent cells after 24-h culture of MSCs (black bars) and HIF-MSCs (gray bars). *P < 0.05; **P < 0.01. SEM, standard error of mean.