FIG. 4.

Increased proliferation of HIF-MSCs is Notch independent, but SUMO1 dependent. MSCs and HIF-MSCs were treated or not with 10 μM RO4929097 (RO) for 48 h (A) or with 50 μM anacardic acid (AA) for 4 h (B), and the proliferation rate was quantified by BrdU incorporation (black bars for MSCs and gray bars for HIF-MSCs). Images were taken at 100 × magnification. Scale bar: 100 μm Data represent mean ± SEM of three independent experiments. *P < 0.05; **P < 0.01. BrdU, 5′-bromo-2′-deoxyuridine; SUMO, small ubiquitin-like modifier.