FIG. 6.

Effects of Prx I levels on the cell death caused by JDA-202. (A) EC109 cells were treated with JDA-202 for 24 h with indicated concentrations, and the expression of Prx I was determined by Western blot. The representative expression (B) and quantitation (C) of Prx I after Prx I siRNA#1, siRNA#2, and siRNA#3 interference for 48 h in EC109 and HET-1A cells. The letters on the left refer to the indicated cells, and the letters on the right refer to the detected proteins. Apoptosis-related cell morphologies (D) and apoptosis analysis and values (E, H) in EC109 cells after downregulation of Prx I combined with or without JDA-202 treatment. Cell morphologies (F) and apoptosis values (G) in HET-1A cells after downregulation of Prx I. (I, J) Overexpression and quantitation of Prx I in EC109 cells. (K, L) Overexpression of Prx I in EC109 cells almost completely reversed cell apoptosis caused by JDA-202. Data are presented as means ± SD. Three individual experiments were performed for each group. **p < 0.01 compared with the controls. ^##p < 0.01, when the JDA-202 plus Prx I OE group is compared with the JDA-202 group. siRNA, short interfering RNA. To see this illustration in color, the reader is referred to the web version of this article at www.liebertpub.com/ars