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Given here are the representative fluorescence decays of 2 μM donor-labeled actin in the absence (black, FD, right) and presence (FD+A) of acceptor-labeled cardiac S1 (10 μM), and in the absence (green, left) and presence (red, middle) of saturating ATP (1 mM). (Top) WT-ELC S1. (Bottom) E56G-ELC S1. Unlabeled S1 had no effect on FD, so faster FDA decays indicate FRET. Signals are normalized to the peak intensity. To see this figure in color, go online.
