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. Author manuscript; available in PMC: 2018 Jul 1.
Published in final edited form as: Acta Biomater. 2017 Apr 5;56:80–90. doi: 10.1016/j.actbio.2017.04.002

Figure 3. Controlled presentation and release of mCherry.

Figure 3

(a) AzTMBmCh was covalently immobilized to the hydrogels for temporally triggered release upon addition of thrombin. Hydrogels with noncleavable AzmCh were created as enzyme-stable, noncleavable controls. (b) Selective release of AzTMBmCh is observed with confocal microscopy (confocal tile images stitched together) over time after thrombin addition, whereas minimal loss of fluorescence from the control AzmCh hydrogel was observed in the presence of thrombin (Scale bar, 1 mm). (c) Fluorescence of these hydrogels was quantified using ImageJ and normalized to t=0 values.