. 2017 May 1;2(1):87–95. doi: 10.1089/can.2016.0032

Table 1.

Methodological Comparison of Five Δ⁹-Tetrahydrocannabinolic Acid A Affinity Studies

	Radioligand	CB species	Expression model	THCA-A source
Rosenthaler et al.²³	[³H]CP55,940	hCB₁	Sf9 cells	THC Pharm GmbH, synthetic, 1 mg/mL in methanol
Verhoeckx et al.²⁴	n.d.^a	hCB₁	Sf9 cells	Extracted from plant material, in ethanol
Ahmed et al.²⁵	[³H]CP55,940	rCB₁	Brain membranes	Extracted from plant material, in hexane
Husni et al.²⁶	[³H]CP55,940	rCB₁	Brain membranes	Extracted from plant material, in hexane
This study	[³H]CP55,940	hCB₁	HEK293 cells	Cayman Chemical, synthetic, 1 mg/mL in acetonitrile

^{^a}

K. Verhoeckx (personal communication, April 2017) reports “outsourcing” the affinity part of their study and could not recall the specific radioligand used in the assay.

hCB₁, human cannabinoid receptor subtype one; HEK, human embryonic kidney; rCB₁, rat cannabinoid receptor subtype one; Sf9, Spodoptera frugiperda clonal isolate 9; THC, Δ⁹-tetrahydrocannabinol; THCA-A, Δ⁹-tetrahydrocannabinolic acid A.