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. 2017 Jul 14;12(7):e0181264. doi: 10.1371/journal.pone.0181264

Fig 5. Halorhodopsin-mediated silencing of the MRR ameliorates acute and remote, but not recent effects.

Fig 5

Mice were submitted to electric shock conditioning either in a regular way or while their MRR was silenced by halorhodopsin illumination by yellow light. Halorhodopsin was expressed in all mice by a viral vector that carried the NpHR gene, and all mice were connected to optic fibers. (A) and (B) The acute effects of electric shocks were partially ameliorated by MRR silencing during the conditioning trial. (C) Effects were not secondary to alterations in pain perception, which was studied in the hot-plate and expressed as the temperature that consistently elicited paw licking. (D) Halorhodopsin silencing did not affect recent conditioned fear 24h after shock-conditioning, but markedly ameliorated remote freezing 7 days later. Note that freezing was statistically similar in MRR-silenced and non-shocked groups. * p<0.05 significant difference from non-shocked; # p<0.05 significant difference from shocked.