Fig 4. Distribution of GABA_A receptor clusters in WT and NL3 KO retina.

To assess the integrity of the inhibitory postsynaptic compartment in the absence of NL3, WT and NL3 KO retinae were labeled for GABA_Aα1, α2, α3, and γ2 receptor subsets (A). A selective but dramatic reduction in the number of GABA_Aα2 receptor clusters was observed in the NL3 KO retina compared to WT (A, B). Of note, the number of the other GABA receptor subsets, Gephyrin and PSD-95 is comparable in WT and NL3 KO retinae. N = 4 WT-KO littermate pairs, at least 5 images per sample. OPL, outer plexiform layer; INL, inner nuclear layer; IPL, inner plexiform layer; GCL, ganglion cell layer.