Fig 1. Overview of the retroduplication calling pipeline.

A—A simplified flow chart of our calling pipeline. B—A schematic diagram of our strategies. We first align unmapped reads to exon junction libraries and use decoy libraries to control the false discovery rate (FDR). Then, we collect discordant paired-end reads, and cluster the reads that are mapped distal to the parent genes. Clustered distal reads indicate retroduplication insertion site.