Fig 3. The ESRE gene network is activated by acute iron removal.
(A) Expression of ESRE-containing genes in worms exposed to 1 mM 1,10-phenanthroline. Fold changes were normalized to vehicle (DMSO) control. (B-C) Venn diagrams of genes upregulated by exposure to 1 mM 1,10-phenanthroline (Chelator) and either P. aeruginosa exposure in liquid (B) or P. aeruginosa infection on agar plates (C). In each case, the number of genes in each category is shown, as is the p-value (based on hypergeometric distribution). (D) Fluorescence microscopy of reporters with (left) or without (right) the ESRE element that were treated with 1 mM 1,10-phenanthroline (Phe) or vehicle (DMSO). Representative images are shown; three biological replicates (with ~50 worms / replicate) were analyzed. A schematic of the promoter region for the construct is shown for convenience. (E) Quantification of GFP fluorescence for a Phsp-16.1::GFP reporter with or without the ESRE element that was treated with vehicle (DMSO) or 1 mM 1,10-phenanthroline (Phe). Error bars represent SEM.