Fig 4. Dectin-1 deficiency further reduces inflammasome activation in the absence of functional Dectin-2 through Syk-JNK signal pathway.

BMDCs from wild type, Dectin-2-deficient (Clec4n^-/-), Dectin-1-deficient (Clec7a^-/-), and Dectin-1 and Dectin-2 double-deficient (Clec7a^-/-Clec4n^-/-) mice were pretreated with (A) or without (B, C and D) anti-Dectin-1 or -Dectin-2 blocking antibody (2 μg/ml) for 1 h before stimulation with H. capsulatum. (A, C and D) Cell-free supernatants, cell lysates and cell pellets were subjected to Western blotting to analyze ASC oligomerization (A), inflammasome components (C) and signaling molecules (D). IgG2a and IgG2b were used as isotype controls. (B) Cell culture supernatants were collected at 18 h after stimulation. Secreted IL-1β was quantified by ELISA (n = 5). Error bars indicate standard deviation of the mean. One representative of two independent experiments is presented. * p < 0.05, ** p < 0.01, *** p < 0.001 [one-way ANOVA with Tukey post-hoc analysis (B)].