Fig 5. Both Dectin-1 and Dectin-2 are important for IL-1β production by CD103⁺ DC in pulmonary H. capsulatum infection.

(A, B and C) Wild type, Clec4n^-/- and Clec7a^-/-Clec4n^-/- mice were intratracheally infected with or without 2.5 × 10⁵ H. capsulatum. Mice were killed on day 7 after infection. Lungs were digested and CD103⁺ DCs, CD11b⁺ DCs, alveolar macrophages (AM) and neutrophils were sorted based on the following markers: CD103⁺ DCs (CD11c⁺CD103⁺CD11b^-), CD11b⁺ DCs (CD11c⁺CD103^-CD11b⁺), alveolar macrophages (Siglec-F⁺F4/80⁺), neutrophils (CD11b⁺Ly6G⁺). Sorted cells were subjected to mRNA extraction. Il1b mRNA expression was analyzed by RT-qPCR (n = 3). Error bars indicate standard deviation of the mean. * p < 0.05, ** p < 0.01, *** p < 0.001, NS, not significant [two-way ANOVA with Sidak post-hoc analysis (A & B) or Tukey post-hoc analysis (C)].