Fig. 2.

Chemical shift perturbations for each residue in the presence of 10–50% v/v [C₄-mim]Br. (a) Combined and weighted ¹H and ¹⁵N (ΔHN) chemical shift perturbations (CSP) of all residues in the presence of 10% (cyan), 25% (green), 40% (orange), 50% (red) v/v [C₄-mim]Br. (b) Cartoon representation of the crystal structure of GB1 (PDB: 2QMT) color coded by amide chemical shifts perturbation (CSP) in 10–50% v/v [C₄-mim]Br. Chemical shift perturbation is indicated by colors ranging from blue (least, 0.00 ppm) to red (most, 0.55 ppm), and the coloring gradient is scaled to the maximum CSP in the 50% [C₄-mim]Br sample. GB1 residues which have more 0.40 ppm CSP in the 50% v/v [C₄-mim]Br sample are identified. The maximum radius of the putty representation is scaled to maximum CSP in the shown condition (10% v/v [C₄-mim]Br, 25% v/v [C₄-mim]Br, 40% v/v [C₄-mim]Br or 50% v/v [C₄-mim]Br). The figure was generated by PYMOL.