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. Author manuscript; available in PMC: 2018 May 23.
Published in final edited form as: Cell Rep. 2017 May 23;19(8):1586–1601. doi: 10.1016/j.celrep.2017.04.069

Figure 1.

Figure 1

Lymphoid and hematopoietic differentiation is impaired in Whsc1+/− and Whsc1−/− cells. (A) Representative FACS plots (out of a total of 10 experiments) of aged WT and Whsc1+/− mice, showing the reduction in the percentages of Whsc1+/− B and T cells. (B) Disadvantage of Whsc1+/− hematopoietic cells in a competitive BM reconstitution assay. The vertical axis shows the percentage of contribution of the indicated Whsc1+/− cell types to the peripheral blood of recipient mice injected with a 1:1 mix of WT:Whsc1+/− cells, as determined by flow cytometry of peripheral blood samples at the times indicated on the “x” axis. Mean ± SEM are shown (C) Percentage of contribution of the indicated Whsc1+/− cell types in recipient mice injected with a 1:1 mix of WT:Whsc1+/− cells, 3–6 months after injection. n = 5 mice for each time point. Mean ± SD are shown. (D) Kinetic delay in the reconstitution of the peripheral blood cellularity by Whsc1−/− cells (red) in comparison with Whsc1+/− (blue) or WT (black) cells, monitored by flow cytometry over 30 weeks after fetal liver transplantation. The vertical axis represents the percentage of donor cells in the peripheral blood of recipient mice at the indicated time points. n= number of mice analyzed. Mean ± SD are shown. (E) Reduced percentage of B cells of Whsc1−/− donor origin in peripheral blood, with time, as indicated in previous panel. Mean ± SD are shown. (F) Reduced absolute numbers of total blood lymphocytes in mice reconstituted with Whsc1−/− donor cells, as measured by automatic hematic biometry. Samples are from mice reconstituted with either Whsc1−/− (red dots), Whsc1+/− (blue diamonds), or WT (black filled squares) cells, 2 months after FLT, or from non-reconstituted, normal age-matched C57Bl/6 control animals (black hollow squares). Mean ± SD are shown. (G) Percentages of the different developmental stages of hematopoietic cells in the BM and spleen from mice reconstituted with either WT or Whsc1−/− cells, 3–6 months after transplant: B cell developmental stages (left graph) and other hematopoietic cell types (right graph). Mean ± SD are shown. (H) Total cellularity of BM and spleen of reconstituted mice. n = number of mice analyzed. Mean ± SEM are shown. See also Figures S1, S2 and S3.