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. 2017 May 18;9(1):122–135. doi: 10.1016/j.stemcr.2017.04.021

Figure 5.

Figure 5

Mechanical Stimuli Induce an Enhanced Cardiac Protein Expression Pattern in hESC-CMs

(A) IF images show expression of MF20 (red), CX43 (green), cTNT (red), and DAPI (blue) in d10 stat and dyn, and d20 stat and dyn hESC-CMs.

(B) Quantification of sarcomere length in d10 and d20 hESC-CMs (n = 20 cells from three independent cultures each). Error bars show SD. N.D. = not detectable. ∗p < 0.01 versus stat hESC-CMs at the same time point.

(C) Relative MFI of cTNT expression in d20 stat and dyn hESC-CMs (n = 4). Error bars show SD. ∗p = 0.0014.

(D) Representative images of d20 stat and dyn hESC-CMs stained with ZR (orange), cTNT (red), and DAPI (blue). ZR/cTNT/DAPI represents an overlay.

(E) Scores of PC-3 and PC-4 of Raman spectra from d20 stat hESC-CMs (n = 30 measurements from three independent cultures) and d20 dyn hESC-CMs (n = 45 measurements from three independent cultures).

(F) PC-4 loadings represent Raman peaks that changed significantly in d20 dyn hESC-CMs when compared with static d20 stat hESC-CMs (see also Figures S4–S6).