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. 2017 Jun 1;9(1):58–66. doi: 10.1016/j.stemcr.2017.05.003

Figure 2.

Figure 2

ABR Controls Centrosome Dynamics

(A) Centrosomes are visualized by γTUBULIN staining (green). Mitosis or interphase is determined by chromosomal staining pattern and morphology (gray).

(B–E) Live imaging analyses of control or dox-treated tet-shABR cells expressing mVenus-CENT2. (B) Snapshots from Movie S1. The distance between centrosomes was measured with 2-min intervals. t = 0 corresponds to separation starting time, defined as a no-return point of bilateral movement. Arrows indicate NEB onsets. (D) The durations from separation initiation to NEB. The y axis corresponds to the red line-gated periods indicated in (C) control (n = 50) and dox-treated cells (n = 36) were analyzed. (E) The distance between centrosomes at the time of NEB. The y axis corresponds to blue line-gated lengths indicated in (C) control (n = 41) and dox-treated cells (n = 32) were analyzed.

The imaging experiments were performed three times. Scale bars represent 10 μm. Error bars in the graphs represent SD (D and E). Statistics: Student's t test (D and E); ∗∗∗p < 0.001 and ∗∗p < 0.01. See also Figure S2 and Movie S1.