Cyrano Displays Dispersed Subcellular Localization and Exhibits Stability in ESCs
(A) smFISH analysis of a representative ESC colony shows Cyrano localization in the nucleus and cytoplasm of ESCs. Nuclei, blue (DAPI). Scale bar, 10 μm.
(B) Quantitation of Cyrano molecules/ESC.
(C) Subcellular fractionation and qRT-PCR confirms Cyrano's presence in the nucleus and cytoplasm.
(D) Assessment of the stability of Cyrano.
Data are from three independent experiments. Error bars represent SEM. See also Figure S1.