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. Author manuscript; available in PMC: 2018 Sep 1.
Published in final edited form as: J Gastroenterol Hepatol. 2017 Sep;32(9):1587–1597. doi: 10.1111/jgh.13731

Figure 2. Effects of tributyrin treatment on TJ protein expression in proximal colon.

Figure 2

Mice were treated as described in Figure 1. Proximal colon was collected and used to prepare RNA or embedded in OCT for histology. A) Claudin-3 (green), occludin (red) and ZO-1(green) were visualized by immunohistochemistry in sections of proximal colon frozen in OCT. A selected area was cropped and enlarged. All images were acquired using a 40× objective. Images are representative of at least replicate images captured per mouse in 4–6 mice per treatment group. B–D) Expression of claudin-1, occludin, and ZO-1 mRNA was detected in the proximal colon of mice using qRT-PCR. Data are the mean ± SEM. Values with different alphabetical superscripts were significantly different from each other, p < 0.05.