Figure 7.

Cortical Astroglia Are Reprogrammed into iNs in the Adult SVZ-RMS-OB

(A–G) Immunostainings showing GFP⁺ CtxAstro nucleofected with ASCL1 30 dpt in the adult SVZ. Example of granular cell-like iNs observed in the OB (A, white arrowheads; magnified in B). Example of iNs found in the anterior RMS (C, yellow arrowhead; magnified in D). Example of GFP⁺ granular cell-like iN expressing GABA (E–G).

(H) Quantification of iNs located in the GCL (white bar) or RMS (gray bar) 30 dpt (Student’s t test, mean ± SEM).

GCL, granule cell layer; RMS, rostral migratory stream; OB, olfactory bulb; ns, not significant. Nuclei are stained with DAPI (blue). n = 3 animals/condition.

Scale bars, 50 μm.