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. 2017 Jun 22;9(1):5–11. doi: 10.1016/j.stemcr.2017.05.027

Figure 2.

Figure 2

Silencing NF-E2 in HSPCs Affects Human HSC Self-Renewal and T Cell Differentiation In Vivo

(A) Scheme of HSPC transduction, injection into mice, and tissue analysis.

(B) Percentage of human cells in the BM (left), blood (middle), and spleen (right) of mice transplanted with transduced HSPCs. Each dot represents an individual mouse (KDCTRL, n = 5; KDNF-E2, n = 5).

(C) CFC assay using hCD45 cells taken from primary mice in (B).

(D) Percentage of human cells in the thymus of mice transplanted with transduced HSPCs. Each dot represents an individual mouse (KDCTRL, n = 5; KDNF-E2, n = 5).

(E) Scheme of T cell differentiation from HSPCs using MS5-DL1 feeder layer.

(F) Percentage of CD4/CD8 positive cells after 5 weeks of HSPC differentiation (n =3).

Error bars indicate the SEM of data from replicate experiments. The significance of the difference between samples was confirmed using unpaired t tests with equal SD. ∗p < 0.05, ∗∗p < 0.001, ∗∗∗p < 0.0001. See also Figure S2.